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Metformin-mediated enhancement of the infiltration of CD8+ T cells into tumor is associated with elevated tumor blood perfusion(PDF)

Journal of Xian Jiaotong University(Medical Sciences)[ISSN:1671-8259/CN:61-1399/R]

Issue:
2018年6期
Page:
821-824
Research Field:
基础研究
Publishing date:

Info

Title:
Metformin-mediated enhancement of the infiltration of CD8+ T cells into tumor is associated with elevated tumor blood perfusion
Author(s):
WANG Ji-chang1 LI Xiong-xiong2 LI Guang-yue3
1. Department of Vascular Surgery; 2. Department of Breast Surgery; 3. Department of Science and Technology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, China
Keywords:
metformin CD8 CD8+ T cell tumor perfusion linear correlation
CLC:

PACS:
R73
DOI:
10.7652/jdyxb201806010
DocumentCode:

Abstract:
ABSTRACT: Objective   To investigate the linear correlation between metformin-induced increase in the number of CD8+ T cells infiltrated into the cancer and metformin-mediated elevation of tumor blood perfusion. Methods   4T1 murine breast cancer cells were orthotopically injected into the fat pad of the 4th breast of BALB/C mice. Nine days later, the mice were randomly divided into the control and metformin groups. Fifteen minutes before extraction, TRITC-conjugated lectin was injected into the tail vein of the mice. Then, the extracted tumor tissues were prepared for frozen sections, which were then stained with anti-CD8 antibody for further immunofluorescent detection. Spearman correlation analysis was performed to observe the correlation between the parameters.Results   Both the infiltration of CD8+ T cells \[(120.8±26.9)/mm2 vs. (67.9±22.9)/mm2\] and lectin-perfused area [(18357.0±5865.0) pixels/μm2 vs. (10233.0±4434.0) pixels/μm2] were significantly higher in metformin-treated group than in the untreated group. In both groups, the number of the infiltrated CD8+ T cells was linearly correlated with lectin-perfused area (control: R=0.884, P=0.007; metformin: R=0.928, P<0.001). Conclusion   Increased tumor perfusion is closely correlated with metformin-induced increase in the infiltration of CD8+ T cells into the tumor.

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Last Update: 2018-11-14