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The expression of SERPINB5 in the process of hepatocellular carcinoma induced by DEN and NMOR in F344 rats(PDF)

Journal of Xian Jiaotong University(Medical Sciences)[ISSN:1671-8259/CN:61-1399/R]

Issue:
2018年6期
Page:
816-820
Research Field:
基础研究
Publishing date:

Info

Title:
The expression of SERPINB5 in the process of hepatocellular carcinoma induced by DEN and NMOR in F344 rats
Author(s):
SUN Hai-feng12 ZHAO Xing-yu3 JING Li2 GUO Ya-huan1 LEI Bao-xia1 CHEN Wen-juan1 ZHAO Zheng1 NAN Ke-jun4 YANG Guang-ning5
1. Department of Oncology, Tumor Hospital of Shaanxi Province, Xi’an 710061; 2. Department of Clinical Medicine, Xi’an Jiaotong University Health Science Center, Xi’an 710061;
3. Otorhinolaryngology, No.521 Hospital of Ordnance Industry, Xi’an 710061; 4. Department of Oncology, The First Affiliated Hospital of Xi’an Jiaotong University Xi’an 710061; 5. Invasive Technology Department, Tumor Hospital of Shaanxi Province, Xi’an 710061, China
Keywords:
SerpinB5 liver cancer model hepatocellular carcinoma
CLC:

PACS:
R735.7
DOI:
10.7652/jdyxb201806009
DocumentCode:

Abstract:
ABSTRACT: Objective    To evaluate the expression of SERPINB5 in the process of hepatocellular carcinogenesis in chemical-induced F344 rat (hepatocellular carcinoma, HCC) model so as to explore the relationship of SERPINB5 with the occurrence and development of HCC. Methods   We used 50 male F344 rats to induce HCC model. They were treated with diethylnirtosamine and N-nitrosomorpholine. Another 30 male F344 rats were used as controls. After 10 rats in model group and 6 in control group were sacrificed every 4 weeks, the pathological changes were evaluated by H&E staining. The expression of SERPINB5 was detected by immunohistochemistry. Results   F344 rats liver cancer was confirmed 16 and 20 weeks after HCC induction by diethylnirtosamine and N-nitrosomorpholine. Moreover, the morphological differences between HCC-induced and control groups were observed from week 4 induction. With the extension of chemical induction, the typical manifestations of hepatitis were shown at week 12. Typical cirrhosis accompanied by micro-invasion of cancer cells was seen at week 16. At week 20, atypia of cancer cells was more obvious and the invasion of cancer cells was large. The expression of SERPINB5 was significantly decreased (P<0.05) compared with that at week 16 of cancer induction. Conclusion   The expression of SERPINB5 is down-regulated in rat liver tissue, suggesting that SERPINB5 is closely related to the occurrence and development of liver carcinoma. Our findings provide new ideas for the early diagnosis and treatment of HCC.

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Last Update: 2018-11-14