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Vanadyl trehalose-induced oxidative stress in mouse gastrointestinal tract and the protective functions of vitamin C and reduced glutathione(PDF)

Journal of Xian Jiaotong University(Medical Sciences)[ISSN:1671-8259/CN:61-1399/R]

Issue:
2018年6期
Page:
810-815
Research Field:
基础研究
Publishing date:

Info

Title:
Vanadyl trehalose-induced oxidative stress in mouse gastrointestinal tract and the protective functions of vitamin C and reduced glutathione
Author(s):
WEI Qian MUHAMMAD Umar JIANG Ping-zhe LI Xiao-dan LIU Qi-qi XING Shu-guang LUO Wen-ya YANG Xing-kai LI Ming-gang
College of Life Science, Nankai University, Tianjin 300071, China
Keywords:
vanadyl trehalose (VT) oxidative stress GSH/GSTs vitamin C reduced glutathione (GSH)
CLC:

PACS:
R57
DOI:
10.7652/jdyxb201806008
DocumentCode:

Abstract:
ABSTRACT: Objective   To investigate the effects of vanadyl trehalose (VT) on oxidative stress and GSH/GSTs pathway gene expression in mouse gastrointestinal tract as well as the protective effects of vitamin C and reduced glutathione (GSH). Methods   Thirty male Kunming mice were randomly divided into five groups: normal control group (group A), VT group (group B), Vc+VT group (group C), GSH +VT group (group D), and Vc+GSH+VT group (group E). The treatment groups were fed with vanadyl trehalose (125mg/kg) every day, while the control group was given the same amount of normal saline daily. Vc and GSH were administered one hour before vanadyl trehalose treatment via gavage. The treatment lasted 15 days. Then, all mice were killed and the stomach and duodenum were isolated. The content of reduced GSH was measured by DTNB method, the activity of glutathione peroxidase was determined by glutathione oxidase method. Real-time fluorescence quantitative PCR was used to detect the expressions of GCLC, GSS, GSR and GSTpi in GSH/GSTs pathway. Results   The content of GSH and GSH-Px activity and the expressions of GCLC, GSS, GSR and GSTpi in the stomach and duodenum in VT-treated group were lower than those in group A (P<0.05). In groups C, D and E the above indicators were significantly improved, but those only in the stomach in group E reached the level of the normal control group. In groups C and D, except for the amount of GSR expressed in the duodenum, there was no significant difference in the other indexes. Conclusion   VT can cause oxidative stress damage to the gastrointestinal tract of mice, which affects GSH content and GSH-Px activity, and interferes with the normal expressions of GSH/GSTs pathway related genes. Exogenous Vc and GSH and the combination of the two can play a certain role in antioxidant protection and reduce the toxicity of VT. The combination of the two is better than one used alone, and the related indicators in the stomach can reach the normal level.

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Last Update: 2018-11-14