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Small intestinal submucosa promotes the proliferation and vascularization of vascular endothelial cells in vitro(PDF)

Journal of Xian Jiaotong University(Medical Sciences)[ISSN:1671-8259/CN:61-1399/R]

Issue:
2018年6期
Page:
805-809
Research Field:
基础研究
Publishing date:

Info

Title:
Small intestinal submucosa promotes the proliferation and vascularization of vascular endothelial cells in vitro
Author(s):
JIAO Zi-zhao1 XUE Wu-jun2 AN Mao-zhu1 FU Hai-xia1 LI Yang2 LI Feng-lou1 SHENG Lian-fen1
1. Department of Blood Purification, the People’s Hospital of Rizhao Affiliated to Jining Medical College, Rizhao 276800; 2. Department of Renal Transplantation, Hospital of Nephropathy, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, China
Keywords:
small intestinal submucosa umbilical vein endothelial cell cell proliferation vascularization
CLC:

PACS:
R318
DOI:
10.7652/jdyxb201806007
DocumentCode:

Abstract:
ABSTRACT: Objective   To investigate the role of small intestinal submucosa (SIS) in promoting the proliferation and vascularization of umbilical vein endothelial cells (UVECs) in vitro. Methods   UVECs were isolated by collagenase digestion method, and then cultured and identified by morphology and immunofluorescence. SIS was prepared by tissue engineering technique; then its components were analyzed by immunohistochemistry and enzyme linked immunosorbent assay (ELISA) methods. The co-culture system of UVECs and SIS was established; UVECs cultured alone were set as the control group. The proliferation and vascularization of UVECs were compared between the two groups. Results   Immunohistochemical test showed that the prepared SIS was mainly composed of the extracellular matrix components type Ⅰ and Ⅲ collagens. The concentrations of vascular endothelial growth factor (VEGF) and basic fibroblast growth factors (b-FGF) in SIS incubation solution detected by ELISA were (48.83±5.67)ng/mL and (72.36±8.71)ng/mL, respectively. When cultured in vitro, there were angiogenesis and high cell density in UVECs and SIS co-cultured group than in UVECs cultured alone group. Growth curve of UVECs obtained by UVECs count showed that the proliferation ability of UVECs in UVECs and SIS co-cultured group was higher than that in the cultured alone group. Growth curve of UVECs showed at days 4 and 5 of logarithmic phase, UVECs count in UVECs and SIS co-cultured group was (7.91±0.42)×104 and (9.15±0.48)×104, while that in the UVECs cultured alone group was (4.26±0.27)×104 and (6.71±0.31)×104(P<0.01). Population doubling time of UVECs in UVECs and SIS co-cultured group was shorter than that in the UVECs cultured alone group (P<0.01). Conclusion   SIS can promote the proliferation and vascularization of UVECs in vitro by its extracellular matrix components type I and III collagens, and its components VEGF and b-FGF.

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Last Update: 2018-11-14