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The protective effect of teapolyphenol on cardiac function in diabetic cardiomyopathy rats and its mechanism(PDF)

Journal of Xian Jiaotong University(Medical Sciences)[ISSN:1671-8259/CN:61-1399/R]

Issue:
2018年6期
Page:
798-804
Research Field:
基础研究
Publishing date:

Info

Title:
The protective effect of teapolyphenol on cardiac function in diabetic cardiomyopathy rats and its mechanism
Author(s):
ZHENG Meng-ying1 LI Yan2 LIU Jing-yu1
1. Department of Respiration, The Third Affiliated Hospital of Jinzhou Medical University, Jinzhou 121000; 2. The First Affiliated Hospital of Xinxiang Medical College, Xinxiang 453100, China
Keywords:
green tea polyphenol autophagy diabetic cardiomyopathy BECN1β-catenin TCF4 MYC GSK3β MTOR pathway
CLC:

PACS:
R852.21
DOI:
10.7652/jdyxb201806006
DocumentCode:

Abstract:
ABSTRACT: Objective   To investigate the protective effect of green tea polyphenol (GTP) on cardiac function in diabetic cardiomyopathy (DCM) rats and its mechanism. Methods   After the model of type 1 diabetes was established by streptozotocin induction in rats, they were randomly divided into the following 5 groups for treatment: NG group (n=15), DM group (n=12), GTP group (n=12), GTP+CQ group (n=11), and CQ group (n=12). NG and DM groups were given gastric lavage of distilled water. GTP group was intragastrically administered with GTP [400mg/(kg·d)] solution. CQ group was intragastrically administered with CQ solution [50mg/(kg·d)]. GTP+CQ group was intragastrically administered with CQ and GTP solution of the same volume. Western blot was used to detect the expression of protein related to β-catenin/TCF4/GSK-3β and MTOR pathways in the left ventricular myocardium. Results   Compared with those in NG group, the LC3-Ⅱ/Ⅰ/ ratio and BECN1 expression were significantly decreased (P<0.05) while SQSTM1 level was significantly increased (P<0.05) in DM group. The LC3-Ⅱ/Ⅰ ratio and BECN1 protein expression level were higher in GTP group than in DM group (P<0.05), while SQSTM1 protein expression level was lower (P<0.05). The protein expressions of β-catenin, TCF4 and MYC were significantly improved in DM group as compared with NG group (P<0.05), and there were higher p-GSK3β/GSK3β and p-MTOR/MTOR ratios in DM group (P<0.05). GTP treatment significantly decreased the protein expressions of β-catenin, TCF4 and MYC, and also decreased p-GSK3β/GSK3β and p-MTOR/MTOR ratios compared with DM group (P<0.05). Conclusion   GTP participates in the protection of heart function in DCM rats by regulating β-catenin/TCF4/GSK-3β and MTOR pathways related to autophagy.

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Last Update: 2018-11-14