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Effects of lncRNA DOCK9-AS2 on the proliferation and migration of papillary thyroid carcinoma cells(PDF)

Journal of Xian Jiaotong University(Medical Sciences)[ISSN:1671-8259/CN:61-1399/R]

Issue:
2018年6期
Page:
784-787
Research Field:
基础研究
Publishing date:

Info

Title:
Effects of lncRNA DOCK9-AS2 on the proliferation and migration of papillary thyroid carcinoma cells
Author(s):
WU Cong-shan12 SI Yan1 TONG Hou-chao1 ZHUANG Xi1 CAI Jing-sheng1 SHEN Mei-ping1
1. Department of Thyroid Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029; 2. Department of General Surgery, The Third Affiliated Hospital of Nanjing Medical University, Nanjing 210000, China
Keywords:
papillary thyroid carcinoma long non-coding RNAs (lncRNA) DOCK9-AS2 proliferation migration
CLC:

PACS:
R736
DOI:
10.7652/jdyxb201806003
DocumentCode:

Abstract:
ABSTRACT: Objective? ?To detect the expression of long non-coding RNAs (lncRNAs) DOCK9-AS2 in papillary thyroid carcinoma (PTC) and its effects on the proliferation and migration of PTC cells.

Methods?? PTC samples and the corresponding adjacent tissues were collected from The First Affiliated Hospital of Nanjing Medical University during June 2016 and June 2017. The qRT-PCR was performed to detected the expression of DOCK9-AS2 in the tissues, and the relationship between DOCK9-AS2 expression and the patients clinicopathological data was analyzed. The cell transfection technology was used to establish the PTC BCPAP cell downregulating DOCK9-AS2 stably. CCK8 was carried out to investigate the effect of DOCK9-AS2 on the proliferation of the BCPAP cells. The wound-healing assay was used to dectect the effect of DOCK9-AS2 on the migration of the BCPAP cells. Results?? The expression of DOCK9-AS2 in PTC tissues (0.0097±0.0044) was significantly higher than that of adjacent tissues (0.0026±0.0010) (P<0.05). The expression of DOCK9-AS2 in PTC tissues was related to lymph node metastasis and extra-thyroid invasion (P<0.05). Down-regulation of DOCK9-AS2 could suppress the proliferation and invasion of the BCPAP cells (P<0.05). Conclusion?? The expression of DOCK9-AS2 in PTC was significantly increased, and downregulating DOCK9-AS2 could inhibit the proliferation and migration of PTC cells.


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Last Update: 2018-11-12